J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 12, 376-377, May 1971
Copyright © 1971 by Lipid Research, Inc.

Extraction of bile acids from rat feces containing cholestyramine

J. D. Manes and D. L. Schneider

Department of Nutritional Research, Mead Johnson Research Center, Evansville, Indiana 47721

The fecal extraction procedure described by Evrard and Janssen (1) was inadequate for the complete extraction of conjugated bile acids from feces containing the bile acid sequestrant, cholestyramine. As judged by gas-liquid chromatographic analysis, substitution of 0.5 n HCl in absolute ethanol for glacial acetic acid allowed for complete recovery (98-104%) of three different conjugated bile salts in the presence of the resin.

Supplementary key words trifluoroacetates • OV-210 • gas-liquid chromatography

Submitted on October 2, 1970
Accepted on December 21, 1970


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