J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Devor, K. A.
Right arrow Articles by Mudd, J. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Devor, K. A.
Right arrow Articles by Mudd, J. B.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Journal of Lipid Research, Vol. 12, 403-411, July 1971
Copyright © 1971 by Lipid Research, Inc.

Biosynthesis of phosphatidylcholine by enzyme preparations from spinach leaves

K. A. Devor and J. B. Mudd

Department of Biochemistry, and Statewide Air Pollution Research Center, University of California, Riverside, California 92502

The enzymic incorporation of choline-1,2-14C from CDP-choline-1,2-14C into phosphatidylcholine by spinach leaf preparations was characterized.

The enzyme catalyzing the incorporation, choline phosphotransferase, had a pH optimum of about 8.0 and required either Mn2+ or Mg2+ as cofactor. The saturation concentration of Mn2+ was 0.3 mm and that for Mg2+ was 13 mm. The Km for CDP-choline was 10 µm. The choline phosphotransferase was inhibited by sulfhydryl reagents. The enzyme was inactivated at 30°C, but this inactivation could be prevented by dithiothreitol and Mn2+. Preincubation of the enzyme with Mn2+ prevented inhibition by sulfhydryl reagents. The incorporation of diglyceride-U-14C into phosphatidylcholine was also studied. The enzyme did not show any diglyceride specificity when exogenous diglyceride was added, indicating that fatty acid distribution in phosphatidylcholine of spinach is not controlled by choline phosphotransferase.

Supplementary key words diglyceride molecular species • CDP-choline • sulfhydryl inhibitors • subcellular distribution

Submitted on August 28, 1970
Accepted on February 11, 1971


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 All ASBMB Journals   Journal of Biological Chemistry 
 Molecular and Cellular Proteomics   ASBMB Today 
Copyright © 1971 by the American Society for Biochemistry and Molecular Biology.