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Journal of Lipid Research, Vol. 12, 466-472, July 1971
Copyright © 1971 by Lipid Research, Inc.

Thin-layer chromatography of ceramides

Karl-Anders Karlsson and Irmin Pascher

Department of Medical Biochemistry, University of Göteborg, Fack, 400 33 Göteborg 33, Sweden

Ceramides with mono-, di-, and trihydroxy long-chain bases, and normal (saturated and unsaturated), branched-chain, and 2-hydroxy fatty acids have been analyzed by thin-layer chromatography. In most cases the compounds were also run as acetates. Borate, arsenite, and silver ions were used as complexing agents, and the effects of number, position, and stereochemistry of hydroxy groups, and of unsaturation, were studied. The results are discussed in view of analysis of natural ceramide species.

Supplementary key words sphingosine • phytosphingosine • dienic long-chain base • normal fatty acids • 2-hydroxy fatty acids • acetyl derivatives • borate chromatography • arsenite chromatography • silver-ion chromatography

Submitted on September 18, 1970
Accepted on February 25, 1971

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