| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Journal of Lipid Research, Vol. 12, 521-530, September 1971
Copyright © 1971 by Lipid Research, Inc.
First Medical Service, Sahlgren's Hospital, University of Gothenburg, Gothenburg, Sweden
Diameters of fat cells in adipose tissue slices, floating in an isoosmolar solution, were measured under a microscope. The slices were obtained from percutaneous biopsies by freeze-cutting after brief formaldehyde fixation. All cells in a given part of the slice were measured, thus avoiding selection. A normal distribution of fat cell diameters could be demonstrated with this method, as has been found with previously described methods. The error of the method was 2.6% for diameter and 8.0% for weight determinations. Storage of adipose tissue at 4°C for 48 hr had no effects on cell size determinations.
Results with this microscopic method were compared with those obtained from a previously described method for automatic determinations of osmium-fixed fat cells. The latter method was slightly modified by using a viscous electrolyte, which prevented sedimentation of large fat cells, and by using sonication to complete cell separation. The methods agreed closely.
A method for calculating mean fat cell weight using osmium-fixed fat cells is described, which makes determinations of sample wet weight and ratio of lipid to wet weight unnecessary.
Supplementary key words electronic determination • osmium tetroxide • sonication • glycerol electrolyte • Celloscope • Coulter Counter
Submitted on January 26, 1971
Accepted on April 9, 1971
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Movassat, D. Bailbe, C. Lubrano-Berthelier, F. Picarel-Blanchot, E. Bertin, J. Mourot, and B. Portha Follow-up of GK rats during prediabetes highlights increased insulin action and fat deposition despite low insulin secretion Am J Physiol Endocrinol Metab, January 1, 2008; 294(1): E168 - E175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Kamel, S. Norgren, K. Strigard, A. Thorne, H. Fakhrai-Rad, J. Galli, and C. Marcus Age-Dependent Regulation of Lipogenesis in Human and Rat Adipocytes J. Clin. Endocrinol. Metab., September 1, 2004; 89(9): 4601 - 4606. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. C. Chen and R. V. Farese Jr. Determination of adipocyte size by computer image analysis J. Lipid Res., June 1, 2002; 43(6): 986 - 989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Morange, H. R. Lijnen, M. C. Alessi, F. Kopp, D. Collen, and I. Juhan-Vague Influence of PAI-1 on Adipose Tissue Growth and Metabolic Parameters in a Murine Model of Diet-Induced Obesity Arterioscler. Thromb. Vasc. Biol., April 1, 2000; 20(4): 1150 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Valet, D. Grujic, J. Wade, M. Ito, M. C. Zingaretti, V. Soloveva, S. R. Ross, R. A. Graves, S. Cinti, M. Lafontan, et al. Expression of Human alpha 2-Adrenergic Receptors in Adipose Tissue of beta 3-Adrenergic Receptor-deficient Mice Promotes Diet-induced Obesity J. Biol. Chem., October 27, 2000; 275(44): 34797 - 34802. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| All ASBMB Journals | Journal of Biological Chemistry |
| Molecular and Cellular Proteomics | ASBMB Today |
