HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gottfried, E. L. Search for Related Content PubMed PubMed Citation Articles by Gottfried, E. L. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 12, 531-537, September 1971
Copyright © 1971 by Lipid Research, Inc.

Lipid patterns in human leukocytes maintained in long-term culture

Eugene L. Gottfried

Department of Medicine, Cornell University Medical College, New York 10021

The lipid composition of leukocytes maintained in long-term culture was examined in order to clarify the role of immaturity in previously observed differences between normal mature leukocytes and leukemic cells. Cell cultures derived from three types of leukocytes were examined: normal lymphocytes, Burkitt lymphoma, and chronic myelocytic leukemia. Lipid extracts were analyzed for total lipid weight, phospholipids, neutral lipids, and glycolipids. Distribution of individual phospholipids was determined by quantitative two-dimensional thin-layer chromatography. The main phospholipids were phosphatidylcholine (51-54%) and phosphatidylethanolamine (24-25%), with smaller amounts of phosphatidylinositol, phosphatidylserine, sphingomyelin, and cardiolipin. All three types of cultured cells showed a remarkable similarity in total phospholipid content (17-18 x 10^-15 moles/cell) as well as in phospholipid distribution. More variation was seen in neutral lipid content. Glycolipid was abundant (17-23% of total lipid weight) and was present mostly as ceramide dihexoside. Compared with normal lymphocytes or polymorphonuclear leukocytes, the cultured cells showed increased phosphatidylcholine, decreased sphingomyelin, and decreased cholesterol content, similar to the changes found in leukemic leukocytes. These findings suggest that the altered lipid patterns found in leukemic leukocytes are a reflection of cell immaturity rather than a characteristic peculiar to the leukemic state.

Supplementary key words tissue culture • Burkitt lymphoma • leukemia • phospholipids • glycosphingolipids • cholesterol • thin-layer chromatography

Submitted on February 11, 1971
Accepted on May 3, 1971

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				M. Tani, T. Sano, M. Ito, and Y. Igarashi Mechanisms of sphingosine and sphingosine 1-phosphate generation in human platelets J. Lipid Res., November 1, 2005; 46(11): 2458 - 2467. [Abstract] [Full Text] [PDF]