Journal of Lipid Research, Vol. 12, 563-569, September 1971
Copyright © 1971 by Lipid Research, Inc.

Four-directional-development thin-layer chromatography of lipids using trimethyl borate

J. Dennis Pollack , Donna S. Clark , and Norman L. Somerson

Department of Medical Microbiology, The Ohio State University, and the Children's Hospital Research Foundation, Children's Hospital, Columbus, Ohio 43205

Solvent mixtures containing trimethyl borate virtually eliminated the pronounced interconversion of 1,2- and 1,3-dipalmitins during their resolution by thin-layer chromatography on Silica Gel G. With trimethyl borate, an average of 1-2% of 1,2-dipalmitin was converted to 1,3-dipalmitin. A four-directional-development TLC procedure incorporating trimethyl borate resolves cholesteryl glucoside, ceramides, monogalactosyl diglyceride, 1- and 2-monopalmitin, palmitic acid, cholesterol, 1,2- and 1,3-dipalmitin, tripalmitin, methyl palmitate, cholesteryl palmitate, ßbeta;-carotene and some of its degradation products, squalene, and tetracosane. Digalactosyl diglyceride, phosphatidic acid, phosphatidylglucose, cerebrosides, and other phospholipids remain near the origin. A mixture containing triolein, 1,2- and 1,3-diolein, 1- and 2- monoolein, oleic acid, and cholesterol was resolved in one dimension. A similar series of palmitic-containing neutral lipids was also resolvable in one dimension. These procedures were applied to the TLC of human sera lipids.

Supplementary key words 1,2-dipalmitin • 1,3-dipalmitin • 2-monopalmitin • 2-monoolein • borate • isomerization

Submitted on July 28, 1970
Accepted on May 14, 1971

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