HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jones, A. L. Articles by Ockner, R. K. Search for Related Content PubMed PubMed Citation Articles by Jones, A. L. Articles by Ockner, R. K. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 12, 580-589, September 1971
Copyright © 1971 by Lipid Research, Inc.

An electron microscopic study of endogenous very low density lipoprotein production in the intestine of rat and man

Albert L. Jones and Robert K. Ockner

Departments of Medicine and Anatomy, University of California Medical Center, San Francisco, California 94122, and the Veterans Administration Hospital, San Francisco, California 94121

Previous studies have shown that in the absence of dietary lipid, intestinal lymph contains endogenous very low density lipoproteins (VLDL) which are identical to those in plasma in size, flotation rate, composition, and electrophoretic mobility. In order to document that these particles are produced in the mucosa of the small intestine itself, electron microscopic studies of rat and human intestinal mucosa were carried out.

Small intestinal absorptive cells from rats fasted and restrained for 48 hr were rich in osmiophilic particles of the size of VLDL (300-1000 A). These particles were present in the endoplasmic reticulum and Golgi apparatus, and in intercellular spaces and lacteals; they were most abundant in mucosa from mid-jejunum. Similar particles were seen in jejunal mucosal biopsy specimens obtained from normal human volunteers after a 40-hr fast. After 6 hr of bile diversion or cholestyramine administration to fasted rats, the VLDL-sized particles virtually disappeared from the mucosa, suggesting that they were produced in the mucosa itself and depended upon the absorption of endogenous intralumenal lipid.

These studies provide further evidence for the production of VLDL in absorptive cells of fasting rat and human intestine, and support the concept that the small intestine is a source of endogenous plasma VLDL.

Supplementary key words bile diversion • bile phospholipids • cholestyramine • endoplasmic reticulum • Golgi apparatus • intestinal absorptive cell • jejunal biopsy • lacteals • osmiophilic particles

Submitted on January 7, 1971
Accepted on May 27, 1971

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				I. J. Cartwright and J. A. Higgins Direct Evidence for a Two-step Assembly of ApoB48-containing Lipoproteins in the Lumen of the Smooth Endoplasmic Reticulum of Rabbit Enterocytes J. Biol. Chem., December 14, 2001; 276(51): 48048 - 48057. [Abstract] [Full Text] [PDF]

				I. J. Cartwright, D. Plonné, and J. A. Higgins Intracellular events in the assembly of chylomicrons in rabbit enterocytes J. Lipid Res., November 1, 2000; 41(11): 1728 - 1739. [Abstract] [Full Text]

				I. J. Cartwright and J. A. Higgins Increased dietary triacylglycerol markedly enhances the ability of isolated rabbit enterocytes to secrete chylomicrons: an effect related to dietary fatty acid composition J. Lipid Res., October 1, 1999; 40(10): 1858 - 1866. [Abstract] [Full Text]

				R. Glickman, J Khorana, and A Kilgore Localization of apolipoprotein B in intestinal epithelial cells Science, September 24, 1976; 193(4259): 1254 - 1255. [Abstract] [PDF]