Journal of Lipid Research, Vol. 12, 622-627, September 1971
Copyright © 1971 by Lipid Research, Inc.

Utilization of fatty acids in perfused hypothermic dog kidney

J. S. Huang , G. L. Downes , and F. O. Belzer

Department of Surgery, University of California, San Francisco, California 94122

Utilization of oleic acid in whole dog kidneys perfused in vitro for 24 hr at 10°C was studied, and the data were correlated with results on the utilization of oleic acid in kidney slices incubated in the same perfusate at 10°C. Kidneys perfused without added oleate lost 35% of their total lipid content and 27% of their phospholipids. Addition of serum albumin-bound oleate to the perfusate prevented the loss of neutral lipid and reduced the loss of phospholipid to 8%. The kidney slices incorporated 29% of the added oleate into lipid and oxidized 3.2% to CO₂.

Oleate apparently largely replaces endogenous fatty acids which are oxidized to meet the energy requirements of the kidney. The loss of phospholipid from the perfused organ is taken as an indication of cell damage, which may be reduced but is not prevented by the addition of oleate to the perfusate.

Supplementary key words oleic acid • oxidation • metabolism

Submitted on February 22, 1971
Accepted on June 2, 1971

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