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Journal of Lipid Research, Vol. 12, 635-636, September 1971
Copyright © 1971 by Lipid Research, Inc.

Loss of lipid to plastic tubing

K. Y. Lee

Department of Physiology, The University of Western Australia, Nedlands, Western Australia, 6009

14C-labeled oleic acid and 3H-labeled monoether in a bile salt solution were perfused through three types of plastic tubing. Large proportions of lipid were lost to the walls of silicone rubber and polyvinyl chloride tubes. The major portion of the lipid lost was recoverable only when chloroform-methanol was perfused through the tubings. On the other hand, very little lipid was lost to the wall of polyethylene tubing. Polyethylene tubing should therefore be used in perfusion studies involving lipid-soluble compounds.

Supplementary key words adsorption • silicone rubber • polyvinyl chloride • chloroform—methanol • polyethylene

Submitted on March 3, 1971
Accepted on May 12, 1971


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