Journal of Lipid Research, Vol. 12, 647-652, November 1971
Copyright © 1971 by Lipid Research, Inc.

Effect of exogenous steroids on sterol synthesis in L-cell mouse fibroblasts

George H. Rothblat and Michele K. Buchko

The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104

A number of steroids have been tested in an L-cell tissue culture system to determine their effects on cellular sterol biosynthesis and cellular growth. Cholesterol, desmosterol, lathosterol, 7-dehydrocholesterol, and cholestanone reduce de novo synthesis and produce only limited toxicity at high concentrations of exogenous sterol. Considerable cellular toxicity is observed when cells are grown in the presence of coprostanol and ⁴-cholestenone. No marked effect on either cell growth or sterol biosynthesis is produced by cholestanol, ßbeta;-sitosterol, stigmasterol, campesterol, ergosterol, cholesteryl oleate, or cholestane.

Supplementary key words cholesterol • desmosterol • feedback inhibition

Submitted on January 28, 1971
Accepted on June 10, 1971

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