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Journal of Lipid Research, Vol. 12, 662-670, November 1971
Copyright © 1971 by Lipid Research, Inc.

Relation of lipid peroxidation to loss of cations trapped in liposomes

Michael E. Leibowitz and Merrill C. Johnson

Water Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C. 20012

Lipid peroxidation and alterations in cation loss have been induced in liposomes by ferrous ion, ascorbic acid, reduced and oxidized glutathione, and gamma radiation. Modifications of these effects by tocopherol and 2,6-di-tert-butyl-4-methylphenol (BHT) were studied when these antioxidants were either incorporated in the membrane or were added to already formed liposomes prior to the addition of the chemical agent or to irradiation.

Lipid peroxidation, as indicated by the thiobarbituric acid test for malonic dialdehyde, did not correlate with alterations in cation loss. The largest amounts of lipid peroxidation induced by ascorbic acid and glutathione were associated with decreased cation loss. Inhibition of Fe²⁺- and radiation-induced lipid peroxidation by antioxidants did not inhibit the associated increase in cation loss. Tocopherol was a more effective antioxidant than BHT when it was incorporated in the membrane, whereas BHT was more effective when it was added to the liposomes after formation.

Supplementary key words ascorbic acid • ferrous ion • glutathione • radiation • membrane antioxidants

Submitted on December 9, 1970
Accepted on June 15, 1971

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