Journal of Lipid Research, Vol. 12, 725-730, November 1971
Copyright © 1971 by Lipid Research, Inc.
Lipogenesis from glucose-2-14C and acetate-1-14C in aorta
Charles F. Howard Jr.
Department of Primate Nutrition, Oregon Regional Primate Research Center, Beaverton, Oregon 97005; and Department of Biochemistry, University of Oregon Medical School, Portland, Oregon 97201
Lipogenesis was measured with glucose-2-14C and acetate-1-14C in the everted aortas of normal and atherosclerotic rabbits. More glucose-2-14C than acetate-1-14C was incorporated into lipids in both the normal and the atherosclerotic aorta.
Radiocarbon from glucose-2-14C appeared mainly in triglycerides and phospholipids with a small amount in cholesteryl esters. Incorporation increased almost threefold with atherosclerosis, most of the radioactivity being in the glycerol moiety; radioactivity was predominantly in carbon 2 of glycerol.
About 70% of the acetate-1-14C incorporated into phospholipids and triglycerides was in the fatty acids, and the remainder was in glyceride-glycerol; 98% of the radioactivity in cholesteryl esters was in the fatty acid moiety. Incorporation into cholesteryl esters was increased most during the development of atherosclerosis.
Fatty acid synthesis was similar from both acetate-1-14C and the 2 carbon unit derived from glucose-2-14C, viz., predominantly de novo synthesis of fatty acids with 14 and 16 carbon atoms, and elongation for those of 18 carbons and longer.
Supplementary key words aortic lipogenesis • lipid degradation
Submitted on June 29, 1970
Revised on April 12, 1971
Accepted on June 28, 1971
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