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Journal of Lipid Research, Vol. 12, 747-759, November 1971
Copyright © 1971 by Lipid Research, Inc.

Glucose: a possible intermediate in the oxidation of the side chain of cholesterol in resting and stimulated rats

M. R. Malinow , N. Baker , Phyllis McLaughlin , and Anne Perley

Oregon Regional Primate Research Center, Beaverton, Oregon 97005; University of Oregon Medical School, Portland, Oregon 97001; Radioisotope Research, Veterans Administration Hospital, Los Angeles, California 90073; and Department of Biological Chemistry, University of California School of Medicine, Los Angeles, California 90024

The effect of repeated muscular contraction on the rate of oxidation of the side chain of cholesterol was studied in anesthetized rats. The animals received an intravenous pulse-label injection of either cholesterol-26-¹⁴C, incorporated into rat plasma lipoproteins, or bicarbonate-¹⁴C. In half the animals of each group, the hind legs were repeatedly stimulated by electrical impulses. A multicompartmental analysis was attempted, based on the disappearance curve of plasma free cholesterol-¹⁴C and on the excretion rate of expired ¹⁴CO₂, as well as on previously reported rates of bile acid and adrenal steroid secretion. The rate of expired ¹⁴CO₂ originating from cholesterol-26-¹⁴C was much less than that predicted by the digital computer analysis; cholesterol degradation could not be evaluated since the data were incompatible with a model that assumes direct oxidation of the side chain to CO₂. A revised model was postulated in which an important fraction of the side chain of cholesterol would be converted to CO₂ only after previous conversion to glucose. Direct measurement of plasma glucose-¹⁴C after the injection of cholesterol-26-¹⁴C supported this hypothesis.

Supplementary key words cholesterol pools • plasma free cholesterol • plasma esterified cholesterol • irreversible disposal • side-chain oxidation • fractional turnover • multicompartmental model • muscular stimulation • exercise • cholesterol-26-¹⁴C • bicarbonate-¹⁴C • respiratory ¹⁴CO₂

Submitted on February 26, 1971
Accepted on June 23, 1971

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