J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 12, 777-780, November 1971
Copyright © 1971 by Lipid Research, Inc.

Determination of the specific radioactivity of fatty acids separated as their methyl esters by gas-liquid chromatography

C. Bishop , R. F. Glascock , Elizabeth M. Newell , and V. A. Welch

National Institute for Research in Dairying, University of Reading, Shinfield, Reading, Berkshire, England

Free or combined 3H-labeled fatty acids are converted to their methyl-14C esters or, if labeled with 14C, to their methyl-3H esters. For a given specific radioactivity of the methyl group, the nuclide ratio in the esters separated by GLC is a direct measure of the specific radioactivity of the fatty acids, and quantitative collection is unnecessary. Methods of methylation with minimum quantities of labeled methanol, and of deriving nuclide ratios from channel ratios in a scintillation spectrometer, are given.

Supplementary key words dual nuclide • recovery indicator

Submitted on February 16, 1971
Accepted on July 1, 1971


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