Journal of Lipid Research, Vol. 13, 119-127, January 1972
Copyright © 1972 by Lipid Research, Inc.

Immunochemical studies of organ and tumor lipids. XIX. Cytolytic action of antibodies directed against cytolipin R

Keizo Inoue , Liselotte Graf , and Maurice M. Rapport

Division of Neuroscience, New York State Psychiatric Institute, and Departments of Biochemistry and Pathology, Columbia University College of Physicians and Surgeons, New York 10032

Rabbit antisera to rat lymphosarcoma contain antibodies that are cytolytic for rat erythrocytes in the presence of complement. The reaction can be inhibited completely by pure cytolipin R showing that (a) immune hemolysis can be mediated through lipid determinants in the membrane, and (b) that cytolipin R determinants are present in the intact erythrocyte membrane and exposed on the surface. Optimal conditions for measurement of cytolysis in this system based on release of ⁵¹Cr are described. Degrees of specificity of a number of different antilymphosarcoma sera are shown, based on inhibition of cytolysis by cytolipin R, cytolipin K, cytolipin H, cytolipin F (F-hapten), glucocerebroside, galactocerebroside, ceramide trisaccharide (cer-glu-gal-gal), and a mixed brain ganglioside preparation. The data suggest that cytolytic antibodies and agglutinating antibodies in these antisera are distinctive despite their common specificity for cytolipin R. Lymphosarcoma cells are more effective than erythrocytes in absorbing cytolytic antibodies.

Supplementary key words haptens • rat erythrocytes • hemagglutinin • ⁵¹Cr • cytotoxicity • lymphosarcoma • membrane

Submitted on June 30, 1971
Accepted on September 16, 1971

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?