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Journal of Lipid Research, Vol. 13, 139-142, January 1972
Copyright © 1972 by Lipid Research, Inc.

A method for fractionation of cerebrosides into classes with different fatty acid compositions

Aureliu J. Acher and Julian N. Kanfer

Eunice K. Shriver Center for Research in Mental Retardation, W. E. Fernald State School, Waltham, Massachusetts 02154, and Neurology Research, J. P. Kennedy Jr. Memorial Laboratories, Massachusetts General Hospital, Boston, Massachusetts 02114

A method is described for the separation of beef brain cerebrosides into three fractions containing different classes of fatty acids: nonhydroxy (I), unsaturated nonhydroxy (II), and hydroxy fatty acid cerebrosides (III). The procedure consists of benzoylation of either crude or purified cerebrosides, followed by column chromatographic separation of benzoylated derivatives containing nonhydroxy acids from those containing hydroxy fatty acids. The benzoyl groups are removed by sodium methoxide-catalyzed transesterification; from the reaction mixtures, fractions I and III precipitate. The fraction II present in mother liquor of I was shown to contain mainly short-chain and unsaturated nonhydroxy fatty acid cerebrosides. The fatty acid composition of each fraction was obtained by gas-liquid chromatography.

Supplementary key words cerebrosides • benzoylation • chromatographic separation • hydroxy fatty acid cerebrosides • nonhydroxy fatty acid cerebrosides • unsaturated nonhydroxy fatty acid cerebrosides

Submitted on April 20, 1971
Accepted on July 22, 1971


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