Journal of Lipid Research, Vol. 13, 69-70, January 1972
Copyright © 1972 by Lipid Research, Inc.

Stimulation of cholesterol 7-hydroxylase by phenobarbital in two strains of rats

S. Shefer , S. Hauser , and E. H. Mosbach

Department of Laboratory Diagnosis, Public Health Research Institute of the City of New York, Inc., and the Bureau of Laboratories, New York 10016

The effect of phenobarbital administration on the in vitro activity of cholesterol 7-hydroxylase was investigated in two strains of rats. In rats of the Wistar strain the daily injection of phenobarbital (100 mg/kg per day ip for 5 days) produced a 33% increase in hepatic microsomal protein and a sixfold stimulation of specific activity of the enzyme. In rats of the Charles River colony (Sprague-Dawley derived) identical treatment with phenobarbital resulted in a 45% increase in hepatic microsomal protein and no change in the specific activity of cholesterol 7-hydroxylase. Plasma phenobarbital concentrations were three to four times greater in the Wistar rats, suggesting that these strains differ also in their capacity to metabolize phenobarbital.

Supplementary key words microsomal enzyme

Submitted on July 6, 1971
Accepted on September 10, 1971

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