J. Lipid Res. Neurobiology of Lipids (ISSN1683-5506)
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Journal of Lipid Research, Vol. 13, 281-282, March 1972
Copyright © 1972 by Lipid Research, Inc.

Preservation of lipid reesterifying enzymes in the microsomal fraction of the rat jejunum

A. Singh and J. B. Rodgers Jr.

Department of Medicine, Albany Medical College, Albany, New York 12208

Studies were performed on methods of storage of rat jejunal tissue that would preserve activities of the lipid reesterifying enzymes, acyl CoA:monoglyceride acyltransferase and fatty acid CoA ligase. Storage at -80°C of microsomes prepared from jejunal mucosa or storage of lyophilized microsomes at -20°C was shown to preserve acyl CoA:monoglyceride acyltransferase very well for a matter of weeks. Preservation of fatty acid CoA ligase activity was adequate with either method, but results were not as good as for the transacylase enzyme.

Submitted on April 30, 1971
Accepted on October 21, 1971


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