Journal of Lipid Research, Vol. 13, 311-316, May 1972
Copyright © 1972 by Lipid Research, Inc.

^8(9),22-Ergostadiene-3ßbeta;-ol, an ergosterol precursor accumulated in wild-type and mutants of yeast

L. W. Parks , F. T. Bond , E. D. Thompson , and P. R. Starr

Department of Microbiology, Oregon State University, Corvallis, Oregon 97331; and Department of Chemistry, Revelle College, University of California at San Diego, LaJolla, California 92037

Whereas wild-type strains of Saccharomyces cerevisiae can synthesize up to 7% dry weight of ergosterol, a polyene-resistant mutant has been obtained which produces no ergosterol. Instead, a C-28 methyl sterol is produced, and it has been identified as ^8(9),22-ergostadiene-3ßbeta;-ol. This sterol is converted to ergosterol by wild-type yeasts and is observed transiently in cells during aerobic adaption of anaerobically grown wild-type yeasts. The new sterol is proposed as an intermediate in ergosterol biosynthesis.

Supplementary key words ergosterol • sterol mutant • yeast sterol • polyene resistance

Submitted on July 15, 1971
Accepted on November 29, 1971

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