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Journal of Lipid Research, Vol. 13, 422-423, May 1972
Copyright © 1972 by Lipid Research, Inc.

Oxalate-silica gel thin-layer system for free 2-hydroxy fatty acids and for fatty acyl coenzyme A

M. David Ullman and Norman S. Radin

Mental Health Research Institute, University of Michigan, Ann Arbor, Michigan 48104

The 2-hydroxy fatty acids tend to yield streaks in thin-layer chromatography on silica gel plates. If potassium oxalate is included with binder-free silica gel, good spots are obtained. Similar difficulties are found in paper chromatography of the fatty acid derivatives of coenzyme A, especially with long-chain acids. The same thin-layer system gives good spots with these compounds.

Supplementary key words 2-hydroxystearoyl coenzyme A • 2-hydroxylignoceroyl coenzyme A

Submitted on September 16, 1971
Accepted on December 16, 1971

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