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Journal of Lipid Research, Vol. 13, 424-426, May 1972
Copyright © 1972 by Lipid Research, Inc.

Rapid assay for hormone-sensitive lipase activity of adipose tissue

Hans Tornqvist , Lennart Krabisch , and Per Belfrage

Division of Physiological Chemistry, Chemical Center, University of Lund, Lund, Sweden

A highly specific and rapid assay for hormone-sensitive lipase activity of rat adipose tissue is described. The method employs emulsified 2,3-di-O-oleyl-[9,10-³H₂]oleoyl glycerol as a substrate; it is very sensitive and is suitable for serial sampling.

Supplementary key words triglyceride analog • glycerol diether ester • fatty acid label

Submitted on October 19, 1970
Revised on November 18, 1971
Accepted on December 7, 1971

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