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Journal of Lipid Research, Vol. 13, 468-476, July 1972
Copyright © 1972 by Lipid Research, Inc.

Base exchange reactions of the phospholipids in rat brain particles

Julian N. Kanfer

Eunice Kennedy Shriver Center for Research in Mental Retardation, W. E. Fernald State School, Waltham, Massachusetts 02154, and Joseph P. Kennedy, Jr. Memorial Laboratories, Massachusetts General Hospital, Boston, Massachusetts 02114

A particulate fraction from rat brain catalyzes the incorporation of [¹⁴C]choline, [¹⁴C]ethanolamine, and l-[¹⁴C]serine into phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine, respectively. The reaction appears to be energy-independent since Mg²⁺, CTP, ATP, and NaF have no stimulatory action. The incorporation is inhibited by EDTA and activated by Ca²⁺. The pH optimum for the incorporation of choline is 9.5, for ethanolamine it is 9.0, and for l-serine it is 8.5. Tris, bicine, and imidazole buffers are inhibitory. The incorporations are inhibited by a variety of structurally related alcohols and are stimulated by isoserine (-hydroxy,ßbeta;-aminopropionic acid).

Supplementary key words phosphatidylserine • phosphatidylcholine • phosphatidylethanolamine • base incorporation • isoserine • Ca²⁺ stimulation • rat brain microsomes

Submitted on December 20, 1971
Accepted on March 21, 1972

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