HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Laine, R. Articles by Rapport, M. M. Search for Related Content PubMed PubMed Citation Articles by Laine, R. Articles by Rapport, M. M. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 13, 519-524, July 1972
Copyright © 1972 by Lipid Research, Inc.

On the structure of cytolipin R, a ceramide tetrahexoside hapten from rat lymphosarcoma

R. Laine , C. C. Sweeley , Y. T. Li , A. Kisic , and M. M. Rapport

Department of Biochemistry, Michigan State University, East Lansing, Michigan 48823

Cytolipin R, a ceramide tetrahexoside isolated from rat lymphosarcoma, was studied by sequential hydrolysis with specific glycosidases which revealed the anomeric configurations of the glycosidic bonds. Sugar linkages were established by combined gas-liquid chromatography and mass spectrometry of the partially methylated alditol acetates prepared after permethylation and hydrolysis of the intact lipid. Results indicated the structure of cytolipin R to be N-acetylgalactosaminyl(ßbeta;13)galactosyl(13)

galactosyl(ßbeta;14)glucosyl ceramide. Cytolipin K (globoside I) differs in having a -galactosyl(14)galactosyl- internal linkage, and this difference must account for the immunological differences between cytolipin K and cytolipin R.

Supplementary key words glycosphingolipid • hapten • cytolipin K • asialo-G_m1-ganglioside • glycosidases • alditol acetates • gas-liquid chromatography • mass spectrometry

Submitted on February 4, 1972
Accepted on April 13, 1972

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				E Nudelman, R Kannagi, S Hakomori, M Parsons, M Lipinski, J Wiels, M Fellous, and T Tursz A glycolipid antigen associated with Burkitt lymphoma defined by a monoclonal antibody Science, April 29, 1983; 220(4596): 509 - 511. [Abstract] [PDF]