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Journal of Lipid Research, Vol. 13, 552-554, July 1972
Copyright © 1972 by Lipid Research, Inc.

Determination of acetyl coenzyme A. Interference by a contaminant in malate dehydrogenase

I. Mulder

Laboratory of Veterinary Biochemistry, University of Utrecht, Utrecht, The Netherlands

Spectrophotometric determinations of acetyl CoA with malate dehydrogenase and citrate synthase are likely to overestimate the amount of acetyl CoA in solutions containing acetoacetyl CoA, since commercial preparations of malate dehydrogenase may contain thiolase.

Supplementary key words acetoacetyl CoA • thiolase

Submitted on July 8, 1971
Accepted on February 22, 1972

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