Journal of Lipid Research, Vol. 13, 716-724, November 1972
Copyright © 1972 by Lipid Research, Inc.

Compartmental and semicompartmental approaches for measuring glucose carbon flux to fatty acids and other products in vivo

Nome Baker and R. J. Huebotter

Radioisotope Research, Veterans Administration, Wadsworth Hospital Center, Los Angeles, California 90073, and Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California 90024

We have attempted to estimate the flux of glucose carbon to total body fatty acids and to other metabolic end products in Bar Harbor 129/J mice fasted 5-8 hr. Tracer [U-¹⁴C]glucose was injected intravenously, and the following data were obtained at various times up to 180 min: plasma glucose C specific activity, plasma glucose concentration, total body glycogen, and ¹⁴C in total body fatty acid, total body lipid, unsaponifiable lipid, expired CO₂, and in hepatic and extrahepatic glycogen. The data were analyzed by three techniques, namely, multicompartmental, semicompartmental, and noncompartmental analyses. All three methods yielded comparable rates of glucose C conversion to total body fatty acids (2-3 µg of glucose C/min/20 g of body weight). Although the semicompartmental approach is extremely simple (it only requires analyses of plasma glucose specific activity as a function of time and ¹⁴C-labeled fatty acid at one point in time), it gives an apparently valid approximation for the flux of glucose C to fatty acids. Other quantitative aspects of glucose metabolism in postabsorptive mice are also considered.

Supplementary key words multicompartmental analysis • [U-¹⁴C]glucose • glycogen • carbon dioxide • noncompartmental analysis • mice

Submitted on March 1, 1972
Accepted on July 3, 1972

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