Journal of Lipid Research, Vol. 13, 802-809, November 1972
Copyright © 1972 by Lipid Research, Inc.

Calculation of lipolysis and esterification from glycerol metabolism in rat adipose tissue

Emilio Herrera and Alejandro Ayanz

Departamento de Endocrinología Experimental, Inst. G. Marañón del C.S.I.C., Velázquez, 144, Madrid-6, Spain

Pieces of epididymal fat pad from fed and 48-hr-fasted rats were incubated for various periods of time in Krebs-Ringer bicarbonate containing [1-¹⁴C]glycerol. The radioactive substrate taken up by the tissue increased linearly with time in both groups and was mainly converted to glyceride-glycerol and CO₂. The slopes of the regressions of ¹⁴C-labeled glyceride-glycerol with time were not different between the groups, while those of ¹⁴CO₂ were smaller in the fasted than in the fed animals. Because the radioactive glycerol in the medium is being continuously diluted with the glycerol coming out of the tissue, it is necessary to take account of this factor in calculating the actual amount of glycerol utilized by the tissue. The glycerol produced by the tissues is higher in the fasted than in the fed animals, and in both groups it increases hyperbolically with time. As negligible amounts of the ¹⁴C-labeled glycerol taken up by the tissue recirculates to the medium, the rates of glycerol release (lipolysis), esterification, and oxidation to CO₂ were calculated.

Supplementary key words glycerol utilization

Submitted on May 16, 1972
Accepted on August 2, 1972

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