Journal of Lipid Research, Vol. 13, 810-815, November 1972
Copyright © 1972 by Lipid Research, Inc.

Rabbit muscle gangliosides

F. E. Lassaga , I. Albarracin de Lassaga , and R. Caputto

Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, Argentina

Four ganglioside fractions were isolated from rabbit muscle: one hematoside and three hexosamine-containing species. They were analyzed for hexoses, hexosamine, sialic acid, fatty acids, and long-chain base content. The molar ratios of sphingosine-hexose-hexosamine-sialic acid were: for hematoside, 1:2:0:1; for the disialogangliosides, 1:3:1:2; and for trisialoganglioside, 1:3:1:3.

The carbohydrates were studied by thin-layer and paper chromatography. The hexoses were glucose and galactose; the hexosamine was N-acetylgalactosamine and the sialic acid was N-acetylneuraminic acid.

Fatty acids and long-chain bases were analyzed by gas-liquid chromatography. The fatty acid composition was similar in all of the four gangliosides. The most abundant fatty acids were 16:0 and 18:0, but significant amounts of 16:1, 18:1, 20:0, and 22:0 were also found. Hydroxy fatty acids were not detected. In all of the muscle gangliosides the main long-chain bases were C₁₈-sphingenine and C₂₀-sphingenine. In hematoside there were also measurable amounts of C₁₈-sphinganine and C₂₀-sphinganine, whereas in the major gangliosides only traces of C₁₈-sphinganine were detected.

Supplementary key words neutral hexoses • N-acetylgalactosamine • N-acetylneuraminic acid • fatty acids • long-chain bases

Submitted on September 8, 1971
Revised on July 18, 1972
Accepted on August 2, 1972

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