J. Lipid Res.
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Journal of Lipid Research, Vol. 13, 816-819, November 1972
Copyright © 1972 by Lipid Research, Inc.

New device for preparing thin slices of adipose tissue for metabolic studies in vitro

J. D. Faulhaber , H. U. Klor , and H. Ditschuneit

University of Ulm, Center of Medicine and Pediatrics, Division of Metabolism and Nutrition, 79 Ulm, Germany

A new microtome is described which allows the rapid preparation of equal slices of well-defined thickness of fresh human tissue, especially adipose tissue. Presetting the microtome for a section thickness of 500 µm, we found a variation of about 5% with human adipose tissue. Slices of human adipose tissue sliced by the microtome showed a higher sensitivity to insulin and a better reproducibility of results than slices prepared freehand.

Supplementary key words fresh-tissue microtome • human adipose tissue • lipogenesis • lipolysis

Submitted on March 24, 1972
Accepted on July 10, 1972


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