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Journal of Lipid Research, Vol. 13, 824-826, November 1972
Copyright © 1972 by Lipid Research, Inc.

A simple lipase assay using trichloroacetic acid

Michael C. Schotz and Arlene S. Garfinkel

Research, Veterans Administration, Wadsworth Hospital Center, Los Angeles, California 90073, and Department of Medicine, UCLA Center for the Health Sciences, Los Angeles, California 90024

An extremely rapid and sensitive assay for lipoprotein lipase activity, suitable for routine determinations, is described. The substrate for the assay is emulsified [2-3H]glyceryl trioleate, activated by serum. The method is based on trichloroacetic acid precipitation of unreacted substrate and measurement of 3H-labeled glycerol.

Supplementary key words adipose tissue • postheparin plasma • triolein • 3H-labeled glycerol • lipoprotein lipase • triglyceride

Submitted on July 27, 1972
Accepted on August 19, 1972


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