Journal of Lipid Research, Vol. 14, 50-53, January 1973
Copyright © 1973 by Lipid Research, Inc.
Bile acid formation in man: metabolism of 7
-hydroxy-4-cholesten-3-one in bile fistula patients
Russell F. Hanson , Peter D. Klein , and Gale C. Williams
Gastroenterology Unit, Department of Medicine, University of Minnesota, Minneapolis, Minnesota 55455, and Division of Biological and Medical Research, Argonne National Laboratory, Argonne, Illinois 60439
7
-Hydroxy-4-cholesten-3-one is thought to be an intermediate in human bile acid synthesis. This conclusion is based on in vivo experiments in animals and on in vitro studies in which homogenates of animal and human livers were used. To further establish that this compound is an intermediate in human bile acid synthesis, its metabolism was studied in subjects with complete bile fistulas. After administration of 3H-labeled 7-hydroxy-4-cholesten-3-one by single intravenous injection, approximately 85% of the administered isotope was recovered in the bile during the first 12 hr. More than 96% of the radioactivity recovered in the bile was identified as either chenodeoxycholic acid or cholic acid, with only a trace amount of the radioactivity present as neutral sterols. This study gives support to the hypothesis that 7-hydroxy-4-cholesten-3-one is a natural intermediate in human bile acid synthesis.
Supplementary key words cholic acid • chenodeoxycholic acid
Submitted on July 24, 1972
Accepted on September 21, 1972
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