Journal of Lipid Research, Vol. 14, 145-151, March 1973
Copyright © 1973 by Lipid Research, Inc.
Effect of CO2 concentration on phospholipid metabolism in the isolated perfused rat lung
William J. Longmore , Carolyn M. Niethe , Deborah J. Sprinkle , and Rodolfo I. Godinez
Department of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri 63104
Studies have been carried out on the incorporation of [U-14C]glucose, [2-14C]pyruvate, [2-14C]acetate, and [1-14C]-palmitate into the phospholipids of the isolated perfused rat lung in the presence of either 6 or 45 mm total CO2 concentration in the perfusion medium. Incorporation of [U-14C]glucose into total phospholipid and into the phosphatidylcholine fraction was increased 19-53% over the 2-hr perfusion period in lungs perfused with medium containing 45 as compared with 6 mm CO2. The incorporation of [2-14C]acetate, [2-14C]-pyruvate, and [1-14C]palmitate was not affected by the change in medium CO2 concentration. Increased incorporation of [U-14C]glucose combined with a shift toward greater incorporation into the fatty acids of the phosphatidylcholine fraction produced a maximum increase of 90% in [U-14C]glucose incorporation into the fatty acids of phosphatidylcholine after 2 hr of perfusion in the presence of medium containing 45 mm CO2 as compared with 6 mm CO2. The increase in medium CO2 concentration produced as much as a 150% increase in [U-14C]glucose incorporation into palmitate derived from the phosphatidylcholine fraction.
The results provide evidence that glucose functions as an important precursor of palmitate in the phosphatidylcholine fraction of lung phospholipids and that the CO2 concentration of the perfusion medium affects the incorporation of glucose into palmitate.
Supplementary key words [U-14C]glucose • [2-14C]pyruvate • [1-14C]palmitate • [1-14C]acetate • phosphatidylcholine
Submitted on June 20, 1972
Accepted on October 30, 1972
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