Journal of Lipid Research, Vol. 14, 160-168, March 1973
Copyright © 1973 by Lipid Research, Inc.
Cellular and enzymatic changes in porcine adipose tissue during growth
D. B. Anderson and R. G. Kauffman
Department of Meat and Animal Science, University of Wisconsin, Madison, Wisconsin 53706
Experiments were designed to define some of the cellular and metabolic changes in various areas of porcine adipose tissue during growth and to establish a relationship between these changes and the accumulation of fat in the domestic pig. 35 male castrate pigs were killed at various ages from late fetal to 6.5 months. The following determinations were made on each animal: (1) total carcass fat, (2) adipose cell size and number by fixation of adipose tissue with osmium tetroxide, and (3) the activities of acetyl CoA carboxylase, citrate cleavage enzyme, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and malic enzyme from perirenal adipose tissue and each of the three layers of subcutaneous backfat. Carcass adipose tissue expanded by a combination of adipocyte hyperplasia and hypertrophy up to 5 months, after which adipose expansion was accomplished by cellular hypertrophy only, with no significant increase in cell number. The activities of the selected lipogenic enzymes (expressed on an adipose cell basis) increased markedly at weaning and again during the rapid increase in percentage of body fat between 3.5 and 5 months. Enzyme activities reached a peak at 5 months, after which activities decreased to values approaching mature levels.
Supplementary key words acetyl CoA carboxylase • citrate cleavage enzyme • malic enzyme • glucose-6-phosphate dehydrogenase • 6-phosphogluconate dehydrogenase • adipocyte size • adipocyte number • fat • growth
Submitted on October 21, 1971
Revised on September 27, 1972
Accepted on November 7, 1972
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