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Journal of Lipid Research, Vol. 14, 224-228, March 1973
Copyright © 1973 by Lipid Research, Inc.

Quantitative analysis of unconjugated and conjugated bile acids in duodenal fluid by densitometry after paper electrophoresis

Neil Kaplowitz and Norman B. Javitt

Gastroenterology Division, Department of Medicine, Cornell University Medical College, New York 10021

A new paper electrophoretic method for the separation of bile acids into five groups, (1) unconjugated, (2) glycine conjugates and (3) taurine conjugates, and (4) and (5) the respective monosulfates, is described. Rapid and accurate qualitative and quantitative estimations of each group are obtained by densitometry after internal standardization and phosphomolybdate color development. The technique can be done in the routine clinical laboratory and is useful for the detection of diseases affecting the enterohepatic circulation of bile acids.

Supplementary key words glycine/taurine ratio • bile acid sulfates

Submitted on June 26, 1972
Accepted on November 6, 1972

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