Journal of Lipid Research, Vol. 14, 229-234, March 1973
Copyright © 1973 by Lipid Research, Inc.

Bile acids. XXXVII. Identification of the 3ßbeta; isomers of allocholic and allochenodeoxycholic acids as metabolites of 5-cholestanol in the rat

Burton W. Noll , Stephen A. Ziller Jr. , E. A. Doisy Jr. , and William H. Elliott

Department of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri 63104

Bile was collected for 18-24 days from adult male rats with cannulated bile ducts that had received intraperitoneally 0.8 mg of 5-[4-¹⁴C, 3-³H]cholestan-3ßbeta;-ol. Bile from the first 2 days containing 14.2% of the administered ¹⁴C and 3.3% of the ³H was hydrolyzed, and the bile acids were separated by acetic acid partition chromatography. The previously unidentified metabolite more polar than cholic and allocholic acids was identified by isotopic dilution as 3ßbeta;,7,12-trihydroxy-5-cholanic acid and represented 3% of the biliary ¹⁴C and 15% of the ³H. Similarly, 3ßbeta;,7-dihydroxy-5-cholanic acid was identified in fractions more polar than allochenodeoxycholic acid and represented 0.6% of the biliary ¹⁴C and 8% of the ³H. More polar fractions contained 4% of the ¹⁴C and 31% of the ³H in unidentified metabolites.

Supplementary key words bile fistula rat • cholestanol metabolism • isomeric allocholanic acid • 5-cholanic acids • 5-[4-¹⁴C, 3-³H]cholestan-3ßbeta;-ol • 3ßbeta;-hydroxy allocholanic acids

Submitted on September 8, 1972
Accepted on November 17, 1972

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