HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Reardon, M. F. Articles by Fidge, N. H. Search for Related Content PubMed PubMed Citation Articles by Reardon, M. F. Articles by Fidge, N. H. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 14, 319-326, May 1973
Copyright © 1973 by Lipid Research, Inc.

Dependence of rates of lipolysis, esterification, and free fatty acid release in isolated fat cells on age, cell size, and nutritional state

Michael F. Reardon , R. Brian Goldrick , and Noel H. Fidge

Department of Clinical Science, The John Curtin School of Medical Research, The Australian National University, Canberra, A.C.T. Australia

Rates of lipolysis, esterification, and free fatty acid release were estimated in isolated epididymal fat cells prepared from rats fed either ad lib. or with a restricted caloric intake. Basal and epinephrine- or theophylline-stimulated rates of lipolysis correlated positively with cell size in the ad lib.-fed group only. Rates of esterification, both basal and epinephrine-stimulated, correlated positively with cell size in the ad lib.-fed group but negatively in the caloric-restricted group. These findings indicate that nutritional factors can modify any possible influence of adipose cell size on lipolysis and esterification. On the other hand, in both groups of rats, epinephrine- and theophylline-stimulated rates of lipolysis correlated positively with the basal rates of lipolysis. Also, rates of esterification in the presence of epinephrine correlated positively with the basal rates of esterification, suggesting that stimulated rates of lipolysis and esterification are at least partly determined by the basal rates regardless of nutritional status. The activity of glycerokinase measured in homogenates of isolated fat cells, if applicable to intact fat cells, was sufficient to cause considerable underestimations of the basal rates of lipolysis (using glycerol production as an index). When lipolysis was stimulated, the potential errors of estimating lipolysis by glycerol production alone were negligible.

Supplementary key words epinephrine • theophylline • epididymal fat pad • glycerokinase

Submitted on May 16, 1972
Accepted on December 27, 1972

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Y.-H. Yu and H. Zhu Chronological changes in metabolism and functions of cultured adipocytes: a hypothesis for cell aging in mature adipocytes Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E402 - E410. [Abstract] [Full Text]