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Journal of Lipid Research, Vol. 14, 364-366, May 1973
Banting and Best Department of Medical Research, University of Toronto, Toronto, Canada, and Departments of Biochemistry and Pediatrics, University of Arkansas Medical Center, Little Rock, Arkansas 72201
A simple, rapid method for the determination of cholesterol in plasma and tissue using o-phthalaldehyde is presented. Comparison of this method with the FeCl3 method gave identical results. However, the o-phthalaldehyde determination is three times more sensitive than the FeCl3 determination (molar extinction coefficients of 11,610 and 33,440 for FeCl3 and o-phthalaldehyde, respectively), it takes less time to complete, and the color developed is more stable. The o-phthalaldehyde method can be used to assay free and esterified cholesterol directly after thin-layer chromatographic separation. Supplementary key words cholesteryl ester • FeCl3 • thin-layer chromatography
Submitted on May 30, 1972
Copyright © 1973 by Lipid Research, Inc.
Determination of cholesterol using o-phthalaldehyde
Accepted on December 12, 1972
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