J. Lipid Res.
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Journal of Lipid Research, Vol. 14, 370-372, May 1973
Copyright © 1973 by Lipid Research, Inc.

A method for the specific labeling of the glycerol in glyceride-containing lipids of Streptococcus faecalis ATCC 9790

Ronald A. Pieringer and Richard T. Ambron

Department of Biochemistry, Temple University School of Medicine, Philadelphia, Pennsylvania 19140

Cells of Streptococcus faecalis incorporate radioactive glycerol exclusively into the glycerol moieties of monoglucosyl diglyceride, diglucosyl diglyceride, phosphatidyl diglucosyl diglyceride, phosphatidylglycerol, diphosphatidylglycerol, phosphatidic acid, diglyceride, and a phospholipid tentatively identified as an amino acyl phosphatidylglycerol. Phosphatidylglycerol is one of the major radioactive lipids synthesized and contains an equal amount of radioactivity in each of its two glycerol moieties.

Supplementary key words glycerol-labeled lipids of S. faecalis • [14C]phosphatidyl glucosyl diglyceride • [14C]diphosphatidylglycerol • [14C]glucosyl diglycerides

Submitted on August 21, 1972
Accepted on January 29, 1973


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