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Journal of Lipid Research, Vol. 14, 385-390, July 1973
Copyright © 1973 by Lipid Research, Inc.

Bile acids. XXXVIII. Conversion of 5-cholestane-3ßbeta;,7-diol to allo bile acids by the rat

Burton W. Noll , E. A. Doisy Jr. , and William H. Elliott

Department of Biochemistry, St. Louis University School of Medicine, St. Louis, Missouri 63104

5-[4-¹⁴C, 3-³H]Cholestane-3ßbeta;,7-diol was prepared from individual samples of 5-[3-³H]cholestane-3ßbeta;,7-diol and 5-[4-¹⁴C]cholestane-3ßbeta;,7-diol, each derived from 3ßbeta;-acetoxycholest-5-en-7-one. Bile was collected for 11 days from adult male rats, with cannulated bile ducts, that had received intraperitoneally 0.90-0.92 mg of the doubly labeled diol. Bile from the first 10 hr, containing 63% of the administered ¹⁴C and 6% of the ³H, was hydrolyzed, and the bile acids were separated by acetic acid partition chromatography. Allochenodeoxycholic and allocholic acids contained at least 20.6% and 48.6%, respectively, of the ¹⁴C retained in the biliary acids. Small amounts of ¹⁴C (2.5% and 1.9%, respectively) were present in the 3ßbeta; isomers of these acids, but the tritium content totaled more than half of that found in the bile acid fraction. No evidence was obtained for presence of the extensive quantities of the allomuricholates.

Supplementary key words 3ßbeta;-hydroxy allo bile acids • allocholic acid • allochenodeoxycholic acid • allomuricholates • 5-[4-¹⁴C]cholestane-3ßbeta;,7-diol • 5-[3-³H]cholestane-3ßbeta;,7-diol

Submitted on October 2, 1972
Accepted on February 5, 1973

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