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Journal of Lipid Research, Vol. 14, 446-458, July 1973
Copyright © 1973 by Lipid Research, Inc.

Metabolic fate of rat and human lipoprotein apoproteins in the rat

Shlomo Eisenberg , Herbert G. Windmueller , and Robert I. Levy

Laboratory of Nutrition and Endocrinology, National Institute of Arthritis, Metabolism, and Digestive Diseases, and Molecular Disease Branch, National Heart and Lung Institute, National Institutes of Health, Bethesda, Maryland 20014

The fate of 125I-labeled apolipoproteins was studied in vivo in rats that had received intravenous injections of 125I-labeled rat HDL and 125I-labeled human HDL, LDL, and VLDL.

Plasma decay curves of rat and human HDL were exponential with similar half-lives in the circulation (11-12 hr). After injection, low molecular weight apolipoproteins (apoLP-alanine of human HDL and fraction HS-3 of rat HDL) were found to redistribute to other lipoproteins, predominantly VLDL. Decay curves of individual HDL proteins were constructed after lipoprotein fractionation, delipidation, and polyacrylamide gel electrophoresis. It was found that the half-lives of the different HDL apoproteins were not identical. A major rat HDL protein (52% of total counts) had a circulating half-life (tfrac12) of 12.5 hr. Two others had a tfrac12 of 8-9 hr while the tfrac12 of several others was 11-12 hr. The tfrac12 of three well-characterized human HDL apoproteins, apoLP-glutamine I, apoLP-glutamine II, and apoLP-alanine, were 13.5, 9.0, and 15.0 hr, respectively.

The fate of 125I-labeled human VLDL and LDL apoproteins in rats was similar to that described previously in humans. After injection of 125I-labeled human VLDL into rats, apoLP-glutamic acid and apoLP-alanine rapidly transferred to rat HDL and were lost thereafter from the circulation from both VLDL and HDL. The apoLDL moiety of human VLDL moved metabolically to the LDL density range (d = 1.019-1.063) through a lipoprotein of intermediate density (d = 1.006-1.019).

Supplementary key words lipoprotein turnover • lipoprotein catabolism • hepatic lipoprotein catabolism • iodinated lipoproteins

Submitted on July 31, 1972
Revised on November 27, 1972
Accepted on March 7, 1973


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