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Journal of Lipid Research, Vol. 14, 563-572, September 1973
Copyright © 1973 by Lipid Research, Inc.

Studies on the formation of C₇-oxygenated cholesterol and ßbeta;-sitosterol metabolites in cell-free preparations of rat liver

Leif Aringer and Peter Eneroth

Department of Chemistry, Karolinska Institutet, and the Hormone Laboratory, Department of Obstetrics and Gynecology, Karolinska Sjukhuset, S-104 01 Stockholm 60, Sweden

The microsomal fraction and the 18,000 g supernatant fluid obtained from livers from normal rats, cholestyraminetreated rats, or from rats with a bile fistula have been used to compare the 7-hydroxylation of [4-¹⁴C]cholesterol and ßbeta;-[4-¹⁴C]sitosterol (24-ethyl-cholesterol). It was not possible to increase the specific formation of 7-hydroxy-ßbeta;-sitosterol above 0.05% with any of the preparations. This conversion was less than 1% of that found for cholesterol. The inhibitory effect of added 7-oxo- and 7ßbeta;-hydroxy-ßbeta;-sitosterol on the 7-hydroxylation of cholesterol was found to be much less than that of the corresponding cholesterol compounds. 7-Hydroxy-ßbeta;-sitosterol was without effect. It is concluded that the activity of the cholesterol 7-hydroxylase is dependent upon the structure of the steroid side chain.

Supplementary key words 7-hydroxylation • cholestyramine • diurnal variations • bile fistula • phenobarbital • inhibition by C₇-oxygenated sterols • interconversion of 7ßbeta;-hydroxy- and 7-oxosterols • epoxides • hydroxyalkylated Sephadex LH-20 • thin-layer chromatography • gas-liquid chromatography-mass spectrometry

Submitted on December 26, 1972
Revised on April 23, 1973
Accepted on May 16, 1973

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