HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Clark, S. B. Articles by Rodgers, J. B. Search for Related Content PubMed PubMed Citation Articles by Clark, S. B. Articles by Rodgers, J. B., Jr. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 14, 581-588, September 1973
Copyright © 1973 by Lipid Research, Inc.

Fat absorption in essential fatty acid deficiency: a model experimental approach to studies of the mechanism of fat malabsorption of unknown etiology

Susanne Bennett Clark , Thomas E. Ekkers , Akbal Singh , John A. Balint , Peter R. Holt , and John B. Rodgers Jr.

Gastrointestinal Division of the Medical Service, St. Luke's Hospital Center, New York 10025, and Department of Medicine, College of Physicians and Surgeons, Columbia University, New York 10032, and the Division of Gastroenterology, Department of Medicine, Albany Medical College of Union University, Albany, New York 12208

Male rats were made deficient in essential fatty acids by feeding them a fat-free diet supplemented with 4% tripalmitin for 8-12 wk from the time of weaning. After feeding 0.5 ml of [¹⁴C]triolein or [³H]oleic acid, 72-hr stool recoveries of radioactivity were significantly greater in deficient rats than in chow-fed controls. Essential fatty acid deficiency did not reduce the absorptive capacities for triolein or for a medium-chain fat, trioctanoin, measured after 3 and 2 hr of maximal-rate duodenal infusion. In everted jejunal slices from essential fatty acid-deficient rats, uptake of micellar [¹⁴C]oleic acid at 0-1°C was similar to that of controls, but the rate of incorporation of fatty acid into triglyceride after rewarming to 37°C was significantly reduced. The specific activities of the microsomal esterifying enzymes, acyl CoA:monoglyceride acyltransferase and fatty acid CoA ligase in jejunal mucosa were 30% lower in essential fatty acid-deficient rats. However, the total microsomal enzyme activity adjusted to constant weight did not differ significantly in deficient rats compared with controls. After intraduodenal perfusion of triolein, accumulation of lipid in the intestinal wall was increased in the deficient rats. Because over 90% of the absorbed mucosal lipid was present as triglyceride, essential fatty acid deficiency appears to affect the synthesis or release of chylomicron lipid from the intestine. Analysis of regions of intestine showed that this delay in transport was most marked in the midportion of the small intestine.

Supplementary key words fatty acid esterification • intestinal fat transport • chylomicron formation • intestinal enzymes

Submitted on December 15, 1972
Revised on April 19, 1973
Accepted on May 29, 1973

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				S. Lukovac, E. L. Los, F. Stellaard, E. H. H. M. Rings, and H. J. Verkade Essential fatty acid deficiency in mice impairs lactose digestion Am J Physiol Gastrointest Liver Physiol, September 1, 2008; 295(3): G605 - G613. [Abstract] [Full Text] [PDF]

				A. Werner, R. Havinga, F. Perton, F. Kuipers, and H. J. Verkade Lymphatic chylomicron size is inversely related to biliary phospholipid secretion in mice Am J Physiol Gastrointest Liver Physiol, June 1, 2006; 290(6): G1177 - G1185. [Abstract] [Full Text] [PDF]

				A. Werner, R. Havinga, T. Bos, V. W. Bloks, F. Kuipers, and H. J. Verkade Essential fatty acid deficiency in mice is associated with hepatic steatosis and secretion of large VLDL particles Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1150 - G1158. [Abstract] [Full Text] [PDF]

				A. Werner, R. Havinga, F. Kuipers, and H. J. Verkade Treatment of EFA deficiency with dietary triglycerides or phospholipids in a murine model of extrahepatic cholestasis Am J Physiol Gastrointest Liver Physiol, May 1, 2004; 286(5): G822 - G832. [Abstract] [Full Text] [PDF]

				A. Werner, D. M. Minich, R. Havinga, V. Bloks, H. Van Goor, F. Kuipers, and H. J. Verkade Fat malabsorption in essential fatty acid-deficient mice is not due to impaired bile formation Am J Physiol Gastrointest Liver Physiol, October 1, 2002; 283(4): G900 - G908. [Abstract] [Full Text] [PDF]

				P. B Jeppesen, C.-E. Hoy, and P. B Mortensen Differences in essential fatty acid requirements by enteral and parenteral routes of administration in patients with fat malabsorption Am. J. Clinical Nutrition, July 1, 1999; 70(1): 78 - 84. [Abstract] [Full Text] [PDF]

				E.-S. Kim, S. K. Noh, and S. I. Koo Marginal Zinc Deficiency Lowers the Lymphatic Absorption of alpha -Tocopherol in Rats J. Nutr., February 1, 1998; 128(2): 265 - 270. [Abstract] [Full Text]