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Journal of Lipid Research, Vol. 14, 589-592, September 1973
Copyright © 1973 by Lipid Research, Inc.

A method for the rapid qualitative and quantitative analysis of 4,4-dimethyl sterols

G. F. Gibbons , K. A. Mitropoulos , and Kalpana Ramananda

Medical Research Council Lipid Metabolism Unit, Hammersmith Hospital, London, W12 OHS, England

A simple and relatively rapid technique has been developed for the separation of several 4,4-dimethyl steryl acetates, some of which contain sterically hindered nuclear double bonds. The method involves thin-layer chromatography on silver nitrate-impregnated silica gel and silver nitrate-impregnated alumina. The separated steryl acetates may then be analyzed quantitatively by gas-liquid chromatography.

Supplementary key words thin-layer chromatography • silica gel-silver nitrate • alumina-silver nitrate • gas-liquid chromatography

Submitted on November 1, 1972
Revised on May 22, 1973
Accepted on June 8, 1973


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