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Journal of Lipid Research, Vol. 14, 592-595, September 1973
Copyright © 1973 by Lipid Research, Inc.

Effects of glycerol on human adipose tissue triglyceride lipase activity

H. Giudicelli and J. Boyer

Laboratoire de la Clinique Endocrinologique, Hôpital de la Conception, 13005, Marseille, France

Glycerol fully protects the human adipose tissue triglyceride lipase against the denaturing effects of high and low temperatures. Under such protection, storage of crude preparations at -10°C or incubation at 50°C resulted in a 1.5-3-fold increase of the measured lipase activity. This increase was shown to be related to enzyme newly released from tissular microparticles present in the samples. Advantage may be taken of these observations to improve greatly the conditions of extraction and storage of this lipase activity.

Supplementary key words heat denaturation • cold denaturation • saccharose • propanediol • extraction • storage

Submitted on September 7, 1972
Accepted on April 25, 1973

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