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Journal of Lipid Research, Vol. 14, 595-598, September 1973
Copyright © 1973 by Lipid Research, Inc.

New sensitive assay for phosphatidylserine decarboxylase based on the detection of CO₂ from nonradiolabeled phosphatidylserine

Thomas G. Warner and Edward A. Dennis

Department of Chemistry, University of California at San Diego, La Jolla, California 92037

A new rapid assay for phosphatidylserine decarboxylase, which is sensitive in the nanomolar range, is described. Synthesis of radiolabeled phosphatidylserine for use as a substrate is not required, since the assay, unlike previous ones, is based on the detection of CO₂ liberated from unlabeled phosphatidylserine. The assay employs a gas chromatographic procedure for the analysis of methane formed by catalytic conversion of the CO₂ produced as a product of the enzymatic reaction.

Supplementary key words phosphatidylserine • carbon dioxide detection • Tetrahymena pyriformis

Submitted on January 29, 1973
Accepted on May 29, 1973

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