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Journal of Lipid Research, Vol. 14, 692-694, November 1973
Copyright © 1973 by Lipid Research, Inc.

Hyocholic acid as internal standard for quantitation of human fecal bile acids

M. T. Ravi Subbiah

Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55901

After hyocholate is added to a fecal homogenate, the bile acids are extracted, purified by thin-layer chromatography, and quantitated by gas-liquid chomatography as methyl ester trifluoroacetates on QF-1 columns.

Supplementary key words gas-liquid chromatography • trifluoroacetates

Submitted on July 17, 1972
Revised on March 20, 1973
Accepted on June 13, 1973


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