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Journal of Lipid Research, Vol. 14, 695-697, November 1973
Copyright © 1973 by Lipid Research, Inc.

New colorimetric method for the quantitative estimation of phospholipids without acid digestion

R. K. Raheja , Charanjit Kaur , Ajit Singh , and I. S. Bhatia

Department of Chemistry and Biochemistry, Punjab Agricultural University, Ludhiana 141004, India

A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described. The phospholipids, after separation by thin-layer chromatography and elution from the silica gel, are heated with a chromogenic solution that is a modification of a spray reagent formulated by Vaskovsky and Kostetsky (1968, J. Lipid Res., 9: 396). The absorbance of the colored complex was read at 710 nm, and it followed Beer's law in the range of 1-10 µg of phospholipid phosphorus.

Supplementary key words chromogenic solution • thin-layer chromatography • egg yolk phospholipids

Submitted on November 15, 1972
Revised on May 4, 1973
Accepted on July 6, 1973

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