HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by O'Brien, J. F. Articles by Geison, R. L. Search for Related Content PubMed PubMed Citation Articles by O'Brien, J. F. Articles by Geison, R. L. Social Bookmarking                      What's this?

Journal of Lipid Research, Vol. 15, 44-49, January 1974
Copyright © 1974 by Lipid Research, Inc.

Incorporation of [2-³H]glycerol into rat brain 1,2-diacyl-sn-glycero-3-phosphorylcholine and 1,2-diacyl-sn-glycerol molecular species in vivo

J. F. O'Brien and R. L. Geison

Waisman Center on Mental Retardation and Human Development, University of Wisconsin, Madison, Wisconsin 53706

Rat brain 1,2-diacyl-sn-glycerols (diglycerides) and 1,2-diacyl-sn-glycerols obtained from 1,2-diacyl-sn-glycero-3-phosphorylcholine after treatment with phospholipase C differ markedly in carbon number distribution. 70% of the 1,2-diacyl-sn-glycerols had a total of 38 fatty acid carbon atoms, and there was no detectable change in the 1,2-diacyl-sn-glycerol mass pattern between 7 and 23 days of age. In contrast, 1,2-diacyl-sn-glycero-3-phosphorylcholine contained at most 10% of this molecular species in the brains of rats of comparable age. A small increase in the C₃₆ species of 1,2-diacyl-sn-glycero-3-phosphorylcholine, which is associated with myelination, was noted between 10 and 17 days. The incorporation of intracranially injected [2-³H]glycerol into 1,2-diacyl-sn-glycero-3-phosphoryl-choline species with polyunsaturated fatty acids containing 20 or 22 carbon atoms was greater than into the species containing only saturated and/or monoenoic fatty acids between 30 min and 24 hr. The 1,2-diacyl-sn-glycerol fractions containing polyunsaturated fatty acids had the lowest specific activity at 30 min. The specific activity of the particular 1,2-diacyl-sn-glycerol fraction containing the stearate-arachidonate pair is the lowest for 4 hr after intracranial injection of the isotope. Thus, molecular species of 1,2-diacyl-sn-glycerol and 1,2-diacyl-sn-glycero-3-phosphorylcholine differed considerably in their labeling patterns, and a direct precursor-product relationship could not be demonstrated during the time period studied.

Supplementary key words CDPcholine:1,2-diglyceride cholinephosphotransferase (EC 2.7.8.2) • diglyceride • lecithin • molecular species biosynthesis • preparative GLC

Submitted on October 19, 1972
Revised on March 28, 1973
Accepted on September 7, 1973

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				Z.-q. Wang, H.-n. Lin, S. Li, and C.-h. Huang Phase Transition Behavior and Molecular Structures of Monounsaturated Phosphatidylcholines J. Biol. Chem., February 3, 1995; 270(5): 2014 - 2023. [Abstract] [Full Text] [PDF]