Journal of Lipid Research, Vol. 15, 65-73, January 1974
Copyright © 1974 by Lipid Research, Inc.

Preparative fractionation of triglyceride mixtures according to acyl carbon number, using hydroxyalkoxypropyl Sephadex

Börje Lindqvist , Inger Sjögren , and Rolf Nordin

Mjölkcentralen, Fack, S-101 10 Stockholm, Sweden

The present paper describes the use of hydroxyalkoxypropyl Sephadex in a liquid chromatography system. When the column is held at 40°C, and when elution is made with a linear gradient of two solvents, an excellent separation of saturated triglycerides in the region C₉-C₅₆ is obtained in 24 hr, even with sample loads as high as 0.5 g/cm² of column.

Triglycerides containing unsaturated fatty acids are eluted more rapidly than their saturated homologs, one C-C double bond being equivalent to -1.42 fatty acid carbon atoms.

Supplementary key words gradient elution • unsaturated triglycerides • reversed phase • hydrophobic gel

Submitted on January 25, 1973
Revised on July 30, 1973
Accepted on September 7, 1973

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