Journal of Lipid Research, Vol. 15, 206-210, May 1974
Copyright © 1974 by Lipid Research, Inc.
Inhibition of free fatty acid mobilization by colchicine
Richard J. Schimmel
Department of Physiology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261
Segments of epididymal adipose tissue from normal male rats were incubated with micromolar concentrations of colchicine for different periods of time up to 4 hr, and the mobilization of free fatty acids (FFA) was measured during a subsequent reincubation. Although pretreatment with colchicine did not alter basal unstimulated FFA release, mobilization of FFA in the presence of epinephrine or theophylline was reduced. However, neither lipolysis, as judged by glycerol production, nor cyclic AMP accumulation was impaired under the same conditions. To assess the possibility that colchicine might limit production of fatty acids by accelerating the entry and metabolism of glucose into adipocytes, the metabolism of glucose by adipose tissue was studied. Pretreatment with colchicine did not affect uptake of glucose nor its oxidation to CO2, although colchicine-treated tissues did have slightly more [14C]glucose incorporated into the glyceride moiety of triglyceride. When adipose tissues pretreated with colchicine were incubated in an albumin-free medium, no reduction in FFA production by colchicine was observed. Because no FFA release occurs in albumin-free media, this experiment suggests that colchicine-induced inhibition of FFA mobilization results from impaired extrusion of FFA from adipose cells.
Supplementary key words lipolysis • glucose oxidation • adipose tissue • microtubules
Submitted on July 18, 1973
Revised on October 19, 1973
Accepted on January 3, 1974
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