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Journal of Lipid Research, Vol. 15, 223-226, May 1974
Copyright © 1974 by Lipid Research, Inc.

High performance liquid chromatography of ceramides: application to analysis in human tissues and demonstration of ceramide excess in Farber's disease

Mutsumi Sugita , Masao Iwamori , James Evans , Robert H. McCluer , John T. Dulaney , and Hugo W. Moser

Eunice Kennedy Shriver Center, Walter E. Fernald State School, Waltham, Massachusetts 02154

Conditions have been determined for the benzoylation of ceramides containing nonhydroxy and hydroxy fatty acids, and a high performance liquid chromatography system for the separation and measurement of these derivatives has been devised that is capable of good resolution and high sensitivity. These methods have been used to determine quantitatively the levels of ceramides in human tissues, and in serum and urine, and to demonstrate elevated amounts of ceramide in Farber's disease urine and tissues.

Supplementary key words fatty acids • benzoylation

Submitted on August 27, 1973
Accepted on December 18, 1973

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